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| Haghighi,Malihe Fathi; Seyyedi,Noorossadat; Farhadi,Ali; Zare,Farahnaz; Kasraian,Leila; Dehbidi,Gholam Reza Refiei; Ranjbaran,Reza; Behzad-Behbahani,Abbas. |
| ABSTRACT Objectives: To investigate the prevalence of human polyomavirus (BK and JC viruses) infection in peripheral blood mononuclear cells of healthy blood donors. Methods: The study included 250 healthy blood donors. Five-milliliter blood was drawn into sterile EDTA tubes and PBMCs were isolated from whole blood. The isolated PBMCs were counted and stored at −70 °C for future investigation. DNA was extracted and subjected to simple, sensitive and specific semi-nested PCR as well as QPCR using both general and specific primers for different assays. Results: Of 250 blood samples, 66 (26.4%) were positive for BKV DNA (146-34,514 copies/106 cells). JC DNA was found in 45 (18%) blood samples (65-21,250 copies/106 cells). Co-infection with these viruses... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood donors; Leukocytes; Mononuclear; Polyomavirus; Real-time polymerase chain reaction. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100022 |
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